Sulfaquinoxaline ELISA monoclonal-antibody kit

This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. SQX residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the SQX residing in it, after comparing with the Standard Curve, multiplied by the dilution multiple, SQX residue quantity in the sample can be calculated.

Kit Components

1.Microtiter plate with 96 wells coated with coupling antigen

2.Standard solutions(6 bottles×1ml/bottle)

0ppb,1ppb,3ppb,9ppb,27ppb,81ppb

3.High concentrated standard control: (1ml/bottle) 1ppm

4.enzyme-labeled secondary antibody solution 7ml red cap

5.Antibody solution 10ml green cap

6.Solution A  7ml white cap

7.Solution B  7ml red cap

8.stop solution  7ml yellow cap

9.20×Concentrated wash solution  40mltransparent cap

10.20×Concentrated extraction solution 50mlblue cap

Sensitivity, accuracy and precision

Test Sensitivity: 1ppb

Detection limit:

Tissue samples:

Sample of high detection limit4ppb

Sample of low detection limit5ppb

Serum and urine samples4ppb

Milk samples20ppb

Accuracy:

Muscle and liver of chicken and pig75±10%

egg69±10%

Honey , milk and serum samples70±10%

Precision:

Variation coefficient of the ELISA kit is less than 10%.