Flumequine ELISA monoclonal-antibody kit

Test Principle        

This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Flumequine residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the tetracycline reside in it, after comparing with the Standard Curve, multiplied by the dilution multiple, Flumequine  residue quantity in the sample can be calculated.

Kit Components

1.Microtiter plate with 96 wells coated with coupling antigen

2.Standard solutions(6 bottles×1/bottle)

    0ppb, 3ppb, 6ppb, 12ppb, 24ppb, 60ppb

3.High concentration standard control:(1ml/bottle)  100ppm

4.enzyme-labeled secondary antibody solution 12ml red cap

5.Antibody working fluid(7ml) green cap

6.Solution A 7mlwhite cap

7.Solution B 7ml red cap

8.Stop solution  7ml yellow cap

9.20×Concentrated wash solution 40ml transparent cap

10.extraction solution  50ml blue cap

Sensitivity, accuracy and precision

Test Sensitivity  0.3ppb

Detection limit

Tissue sample(chicken, chicken liver, pork, pig liver, shrimp and fish, etc.) 0.25ppb

Accuracy

Tissue sample(chicken, chicken liver, pork, pig liver, shrimp and fish, etc.) 85±25%

Precision

Variation coefficient of the ELISA kit is less than 10%.