Enrofloxacin ELISA monoclonal-antibody kit

Test Principle        

This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. Enrofloxacin residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the Enrofloxacin reside in it, after comparing with the Standard Curve, multiplied by the dilution multiple, Enrofloxacin residue quantity in the sample can be calculated.

Kit Components

1.Microtiter plate with 96 wells coated with coupling antigen

2.Standard solutions(6 bottles×1ml/bottle)

0ppb,0.5ppb,1.5ppb,4.5ppb,13.5ppb,40.5ppb

3.High concentration standard control:(1ml/bottle)  1ppm

4.enzyme-labeled secondary antibody solution  7ml red cap

5.Antibody working fluid   7ml green cap

6.Solution A      7ml white cap

7.Solution B      7ml red cap

8.Stop solution    7ml yellow cap

9.20×Concentrated wash solution 40ml transparent cap

10.2×concentrated extraction solution 50ml blue cap

Sensitivity, accuracy and precision

Test Sensitivity   0.5ppb

Detection limit

Tissue sample

High detection limit1ppb

Low detection limit20ppb

Serum1ppb

Honey2ppb

Accuracy

Tissue sample (chicken, chicken liver, pork, pig liver, shrimp and fish, etc.) 85±20%

Serum sample 80±20%

Honey sample 75±15%

Precision

Variation coefficient of the ELISA kit is less than 10%.