Sell Furazolidone ELISA monoclonal-antibody kit(AOZ)

This ELISA kit is designed to detect furazolidone metabolite (AOZ) based on the principle of indirect-competitive enzyme immunoassay. The microtiter wells are coated with capture BSA-linked antigen. AOZ in sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of horseradish peroxidase labeled anti-antibody, tetramethyl-benzidine (TMB)/peroxide substrate is used and the signal is measured by spectrophotometer. The absorption is inversely proportional to the AOZ concentration in the sample.

Kit Components

1.Microtiter plate with 96 wells coated with coupling antigen

2. Standard solutions (6 bottles×1ml / bottle)

   0ppb,0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb       3.High concentration standard control : (1ml/bottle)  100ppb

4.enzyme-labeled secondary antibody solution 12mlred cap

5. concentrated antibody solution 0.8mlgreen cap

6. solution  A  7ml white cap

7. solution  B  7ml red cap

8. stop solution  7ml yellow cap

9. 20×concentrated wash solution 40ml transparent cap

10. 2×concentrated extraction solution 50ml blue cap

11. dinitrobenzal-dehyde 15.1mg black cap

Test Sensitivity: 0.05ppb

Detection limit:

Tissue and honey sample0.1ppb

Egg sample0.1ppb

Milk sample0.1ppb

Accuracy:

Tissue samples(muscle and liver) 75±15%

honey sample 90±15%

Egg sample 90±20%

Milk sample 90±10%

Precision:

Variation coefficient of the ELISA kit is less than 10%.